Cyanobacteria are photosynthetic prokaryotes that live in aquatic environments. The cyanobacteria Synechococcus elongatus coordinates its day and night behaviors via a three protein post-transcriptional oscillator. The oscillator is entrained by light/dark cycles but continues to run in constant conditions. The oscillator finely controls the activity of almost all the organism’s promoters allowing genes to be maximally expressed at particular phases during the day/night cycle. Although the post-transcriptional oscillator has been recapitulated in-vitro, the mechanism that couples the oscillator to gene expression is unknown.
I am interested in studying circadian gene expression in Synechococcus elongatus from a systems perspective. By monitoring global gene expression and protein state, I hope to understand how and why gene expression is under circadian control.
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