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O'Shea Lab > People > Joonhyuk Choi

Direct measurement of intracellular phosphate concentration with FRET (fluorescence resonance energy transfer)
Joonhyuk Choi <choi3@fas>
One of our ultimate goals is to understand how Saccharomyces cerevisiae regulates its phosphate homeostasis pathway at the systems level. In order to accomplish this goal, it is crucial to be able to correlate fluctuations of extracellular phosphate concentration with the change of intracellular phosphate concentration because the PHO pathway essentially depends on the change of intracellular phosphate concentration. If we can measure it directly in a nondestructive and real-time way, we expect to elucidate the general principles to explain not only the interesting static properties such as differences in thresholds of expression of genes and bistability but also the dynamics of the PHO pathway. In this respect, we are trying to utilize a biosensor to detect phosphate concentration in vivo with FRET.


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