O'Shea Lab > People > Dennis Wykoff
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| Regulation of positive
feedback of the PHO pathway in S.
cerevisiae |
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| While studying phosphate uptake in budding yeast,
we noticed an apparent contradiction. Cells inactivated
for Pho84, the high-affinity phosphate transporter
in yeast, and grown in high phosphate behaved as
if they were starved for phosphate (the PHO responsive
genes were induced); however, the Pho84 protein
was not present during growth in high phosphate
conditions. The pho84D strain behaves as if it
is constitutively starving for phosphate but it
is unable to grow in low-phosphate conditions,
suggesting that Pho84 is required to survive phosphate
starvation and that Pho84 is a negative feedback
element. I hypothesized that there is a positive
feedback element regulating the PHO system, and
only when the negative feedback is removed (i.e.,
pho84D cells) does the positive
feedback become apparent. I am currently characterizing
the mechanism
of positive feedback in the PHO signal transduction
pathway. |
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