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Bulk electroporation can be used to transfect several neurons in intact tadpoles with a protein of your choice (GFP in this case). Neurons can then be imaged in-vivo for several days and can in addition be targeted for patch-clamp recordings. This makes it possible to examine in detail the functional and morphological changes of individual neurons during the course of development as well as before and after the application of certain learning paradigms. |
